GC-MS Analysis of Mentha arvensis L. Essential Oil for its Antimicrobial Activity by Bio-autography Assay

The extraction of essential oil for its analysis and anti-microbial study has been in practice from so many years. The present work in Mentha arvensis L. for its anti-bacterial activity using bioautographic technique is for the first time. The essential oil extracted from Mentha arvensis L. was investigated for its antimicrobial activities against Aspergillus niger, Klebsiella pneumoniae and Escherichia coli. The essential oil extracted from the sample was analyzed using GC-MS technique to find out the presence of constituents. There are 72 identified bioactive molecules were identified in which menthol was present predominantly followed by isomenthane, menthol acetate and D-Limonene. The sensitivity of the chemical compounds against the microorganisms was determined by the bio-autographic assay. The oil exhibited maximum zone of inhibition as anti-bacterial agent against Klebsiella pneumoniae and Escherichia coli followed by as anti-fungal agent against Aspergillus niger. The anti-microbial activity could be due to the combined activity of the compounds present in the oil mixture extracted from the sample.

@article{savitha2026,
  author  = {Savitha M Murthy* and Ranjitha M C},
  title   = {{GC-MS Analysis of Mentha arvensis L. Essential Oil for its Antimicrobial Activity by Bio-autography Assay}},
  journal = {Journal of Bioresearch},
  year    = {2026},
  volume  = {3},
  number  = {1},
  pages   = {38-46},
  doi     = {https://doi.org/10.56716/4/2279},
}