Biopesticide for controlling Citrus limon (L.) Burm f. (Assam lemon) pests and diseases

A biopesticide has been developed from Azadirachta indica A. Juss, Polygonum glabrum Willd., Cyclosorus parasiticus (L.) Farw., Curcuma longa L., Capsicum annum L. and cow urine to control insects and pests infestation on Citrus limon (L.) Burm f. (Assam lemon) grown at Kokrajhar district, BTR, Assam. Those five number of locally available medicinal plants selected for pesticide preparation were ground collectively into a fine paste using a mixer grinder along with distilled water. The paste was then sieved with a muslin cloth and squeezed to extract the solvent and diluted up to 200 ml. The resulting filtrate constitutes 50% stock solution later diluted into 1%, 5% and 10% and each solution has been mixed with 200 ml of cow urine before spray. The strength of aqueous extracts were evaluated by foliar spray on plants and their efficacy were determined for controlling the pests and diseases. Meanwhile four pest infested lemon trees were selected and tagged S₁, S₂, S₃ and S₄. Plant tagged as S₁, S₂ and S₃ were considered for applying biopesticide @ 1%, 5% and 10% while S₄ treated as a control plant. The said biopesticide of three different concentrations were sprayed once in a week from January 2024 to March 2025 with a hand sprayer (2L capacity). The biopesticide @10% (S₃) concentration showed the best result with reduction of Diaphorina citri (Citrus psylla), Dialeurodes citri (white fly), Anoplophora versteegi (trunk borer), Phyllocnistis citrella (leaf miner) and other insect population as compared to the lemon plants - S₁, S₂ and S₄.

@article{miniswrang2026,
  author  = {Miniswrang Borgoyary1, Hayen Machahary1, Nutai Gwra Narzary2, Pforeni Jane Sapruna1 and Mohesh Gogoi3*},
  title   = {{Biopesticide for controlling Citrus limon (L.) Burm f. (Assam lemon) pests and diseases}},
  journal = {Journal of Bioresearch},
  year    = {2026},
  volume  = {4},
  number  = {2},
  pages   = {44-51},
  doi     = {https://doi.org/10.5281/zenodo.15393038},
}